CuGenDBv2

Gene ID	Spg033735
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Transcription initiation factor IIF subunit beta
Genome location	scaffold13:38437973..38446369
RNA-Seq Expression	Spg033735
Synteny	Spg033735
Gene Ontology terms	GO:0006367 - transcription initiation from RNA polymerase II promoter (biological process) GO:0006413 - translational initiation (biological process) GO:0005674 - transcription factor TFIIF complex (cellular component) GO:0003677 - DNA binding (molecular function) GO:0003743 - translation initiation factor activity (molecular function) GO:0008168 - methyltransferase activity (molecular function)
InterPro domains	IPR003196 - Transcription initiation factor IIF, beta subunit IPR011039 - Transcription Factor IIF, Rap30/Rap74, interaction IPR036388 - Winged helix-like DNA-binding domain superfamily IPR036390 - Winged helix DNA-binding domain superfamily IPR040450 - TFIIF beta subunit, HTH domain

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_004152667.1 transcription initiation factor IIF subunit beta [Cucumis sativus]

1.8e-129

85.81

Show/hide

Query:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET
        MEDEHG GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSD LPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTET
Subjt:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET

Query:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV
        GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSKEKKKV
Subjt:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV

Query:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

XP_008444765.1 PREDICTED: transcription initiation factor IIF subunit beta-like [Cucumis melo]

4.7e-130

85.81

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Query:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET
        MED+HG GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTET
Subjt:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET

Query:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV
        GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSKEKKKV
Subjt:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV

Query:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

XP_022951326.1 transcription initiation factor IIF subunit beta [Cucurbita moschata]

6.8e-129

84.38

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Query:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG
        MEDE+GGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTETG
Subjt:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG

Query:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA
        NVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSK+K+KVA
Subjt:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA

Query:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        PVKQ +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGG+
Subjt:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

XP_023002475.1 transcription initiation factor IIF subunit beta [Cucurbita maxima]

3.0e-129

84.72

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Query:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG
        MEDE+GGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTETG
Subjt:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG

Query:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA
        NVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSK+K+KVA
Subjt:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA

Query:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        PVKQ +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

XP_038884084.1 transcription initiation factor IIF subunit beta-like [Benincasa hispida]

1.2e-130

85.76

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Query:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG
        MEDE+GGGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTETG
Subjt:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG

Query:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA
        NVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSKEKKKVA
Subjt:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA

Query:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LRL0 Transcription initiation factor IIF subunit beta

8.6e-130

85.81

Show/hide

Query:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET
        MEDEHG GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSD LPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTET
Subjt:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET

Query:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV
        GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSKEKKKV
Subjt:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV

Query:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

A0A1S3BBV5 Transcription initiation factor IIF subunit beta

2.3e-130

85.81

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Query:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET
        MED+HG GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTET
Subjt:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET

Query:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV
        GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSKEKKKV
Subjt:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV

Query:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

A0A5A7VAJ9 Transcription initiation factor IIF subunit beta

2.3e-130

85.81

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Query:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET
        MED+HG GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTET
Subjt:  MEDEHG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTET

Query:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV
        GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSKEKKKV
Subjt:  GNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKV

Query:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  APVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

A0A6J1GH97 Transcription initiation factor IIF subunit beta

3.3e-129

84.38

Show/hide

Query:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG
        MEDE+GGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTETG
Subjt:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG

Query:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA
        NVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSK+K+KVA
Subjt:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA

Query:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        PVKQ +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGG+
Subjt:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

A0A6J1KP21 Transcription initiation factor IIF subunit beta

1.5e-129

84.72

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Query:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG
        MEDE+GGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQ                        FKMEMAGTETG
Subjt:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETG

Query:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA
        NVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM       VIDNDRGVHMRPMPGM+GLIS+TSK+K+KVA
Subjt:  NVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVA

Query:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
        PVKQ +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPA     QFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE
Subjt:  PVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

SwissProt top hits

e value

%identity

Alignment

P13984 General transcription factor IIF subunit 2

8.3e-13

27.68

Show/hide

Query:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF
        +L   K +  VWL+K P  +++ W     S    + K  L +   Q     S  + +D++ + ++                       G  P S S    
Subjt:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF

Query:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK
          FV        + VF+E+S  K+S+EG V  + + +P  SEN     +L  E ++K + +    D+ V     P      +   + KKK       D K
Subjt:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK

Query:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK
        R R D+  + D++F  FE+   + LK LV  T QP V     +LKEIL E+ V N +G ++ T+ELKPEY+
Subjt:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK

P41900 General transcription factor IIF subunit 2

3.7e-13

24.55

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Query:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPS----------DPLPLAKVILSLDP----LQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGT
        +LD   A R VWL+K P  +A+ W+  P +           P   A+V LSL P    L  +E    +   D+S +   ++GV     F  +   +    
Subjt:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPS----------DPLPLAKVILSLDP----LQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGT

Query:  ETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSMVIDNDRGVHMRPMPGMIGLISTTSKEKKKV--APV
         T +                   ++    K+ MEG++  K + +P ++N   Y KL  E   K+    ++    ++P+  ++            +     
Subjt:  ETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSMVIDNDRGVHMRPMPGMIGLISTTSKEKKKV--APV

Query:  KQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK
        K+++ K+ R D+  + D++F  FE+   + +K LV+ T+QP     I +LKEIL ++C YN +  ++  +ELK EY+
Subjt:  KQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK

Q01750 General transcription factor IIF subunit 2

2.9e-13

26.2

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Query:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF
        +L   K +  VWL+K P  +++ W   P    +   ++  +    Q     S  + +D++ + ++                       G  P S S    
Subjt:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF

Query:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK
          FV        + VF+E+S  K+S+EG V  + + +P  SEN     +L  E ++K + +    D+ V     P      +   + KKK       D K
Subjt:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK

Query:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK
        R R D+  + D++F  FE+   + LK LV  T QP     + +LKEIL E+ + N +G ++ T+ELKPEY+
Subjt:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK

Q2T9L9 General transcription factor IIF subunit 2

6.4e-13

25.83

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Query:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF
        +L   K +  VWL+K P  +++ W   P    +   ++  +    Q     S  + +D++ + ++                       G  P S S    
Subjt:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF

Query:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK
          FV        + VF+E+S  K+S+EG V  + + +P  +EN     +L  E ++K + +    D+ V     P      +   + KKK       D K
Subjt:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK

Query:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK
        R R D+  + D++F  FE+   + LK LV  T QP     + +LK+IL E+ V N +G ++ T+ELKPEY+
Subjt:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK

Q8R0A0 General transcription factor IIF subunit 2

2.4e-12

26.57

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Query:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF
        +L   K +  VWL+K P  +++ W     S    + K+ ++ +  +++ S +L   +D++ + ++                       G  P S S    
Subjt:  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNMF

Query:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK
          FV        + VF+E+S  K+S+EG V  + + +P  SEN     +L  E ++K + +    D+ V     P      +   + KKK       D K
Subjt:  KDFV-------PMCVFSEASQGKVSMEGKVEHKFDMKP-HSENLEMYGKLCRERTNKSMVIDN--DRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVK

Query:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK
        R R D+  + D++F  FE+   + LK LV  T QP     + +LKEIL E+ + N +G ++ T+ELKPEY+
Subjt:  RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYK

Arabidopsis top hits

e value

%identity

Alignment

AT1G75510.1 Transcription initiation factor IIF, beta subunit

5.2e-87

58.39

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Query:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSW--------QSHPPSD-PLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFK
        MED H      NLD  K+DRS+WLMKCP++V K+W         S   SD P  +AK++  +DPL+ D                              FK
Subjt:  MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSW--------QSHPPSD-PLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFK

Query:  MEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLIST
        M M G E GN+PK ++LNMF DFVPM  FS+ +QG  + EGKV+HKFDMKP+ E +E Y +LCRERT+K+M       VIDNDRGVHMRPMPGM+GL+S+
Subjt:  MEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLIST

Query:  TSKEKKKVAPVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVE-DTGGE
         SKEK+K  PVKQ++VKRTRRDRGELE IMFKLFE QPNW LKQLVQETDQPA     QFLKEILNELCVYNKRG+NQGTYELKPEYKKS E DTGG+
Subjt:  TSKEKKKVAPVKQSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVE-DTGGE

AT3G52270.1 Transcription initiation factor IIF, beta subunit

6.8e-71

51.43

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Query:  LDTGKADRSVWLMKCPLLVAKSWQSHP-------PSDPL-PLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPK
        L+TG A+RS+ LMK P LVA S QSH        P DP  P AKVIL +DPL  ++  +                          F ME+A  ++GN+P+
Subjt:  LDTGKADRSVWLMKCPLLVAKSWQSHP-------PSDPL-PLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPK

Query:  SFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVAPVKQ
         ++L+M KDF+PM VF E+S GK+S+EGK+++KFDM+PH+EN+E YG+LCRERTNK M       VIDN RG+HMRPMPGMI  I T + EKKK+   + 
Subjt:  SFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSM-------VIDNDRGVHMRPMPGMIGLISTTSKEKKKVAPVKQ

Query:  SDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVED
        S++KRTRRDR E+E++MF LFERQ NW L+ L+QETDQP      QFLK++L +LC+YN +G+NQGTYELKPEYKK+ ++
Subjt:  SDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVED

Sequences

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CDS sequence

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ATGGAAGACGAGCACGGCGGCGGTAGTAGCAGTAACTTGGATACAGGGAAGGCGGATAGATCAGTGTGGCTGATGAAGTGCCCGTTGCTGGTCGCCAAGTCATGGCAGTC
TCATCCTCCATCCGATCCTCTTCCTCTCGCTAAGGTCATCCTTTCTCTCGATCCTCTTCAATCCGACGAGTCCTCTTCTCTCCAGGTTGCAAAAGATATGTCCTACTTGG
ATAATGTCTCCATTGGTGTTTGCTGTGAAACCTTGTTTATGGCTTTGTTTAAGATGGAGATGGCTGGAACTGAGACTGGAAACGTGCCAAAAAGTTTTTCCTTAAATATG
TTTAAAGACTTCGTTCCTATGTGTGTCTTTTCAGAGGCAAGTCAAGGTAAGGTGTCAATGGAAGGGAAAGTGGAACATAAATTCGATATGAAGCCTCATAGTGAAAATCT
TGAAATGTATGGGAAATTGTGCCGTGAAAGGACAAACAAATCCATGGTGATAGACAATGACCGTGGAGTGCATATGAGGCCAATGCCTGGCATGATTGGTTTGATCAGTA
CAACTTCAAAGGAAAAGAAGAAGGTGGCGCCAGTTAAACAATCAGACGTAAAAAGAACTAGAAGGGATCGTGGGGAACTGGAAGATATAATGTTCAAGCTATTTGAAAGA
CAGCCAAATTGGGCCCTAAAGCAGCTTGTCCAAGAGACTGATCAACCTGCAGTATGTCTACTTATCCAATTCTTGAAAGAGATACTGAATGAGCTTTGTGTCTACAACAA
GAGAGGGACTAATCAAGGAACCTATGAGCTTAAACCCGAATACAAGAAATCTGTCGAGGATACCGGCGGAGAGTAG

mRNA sequence

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Protein sequence

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MEDEHGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQVAKDMSYLDNVSIGVCCETLFMALFKMEMAGTETGNVPKSFSLNM
FKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSMVIDNDRGVHMRPMPGMIGLISTTSKEKKKVAPVKQSDVKRTRRDRGELEDIMFKLFER
QPNWALKQLVQETDQPAVCLLIQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGE

Spg033735 (gene) of Sponge gourd (cylindrica) v1 genome