CuGenDBv2

Gene ID	Spg034913
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA (Guanine(10)-N2)-methyltransferase homolog
Genome location	scaffold3:24554979..24561020
RNA-Seq Expression	Spg034913
Synteny	Spg034913
Gene Ontology terms	GO:0001510 - RNA methylation (biological process) GO:0006306 - DNA methylation (biological process) GO:0034470 - ncRNA processing (biological process) GO:0003677 - DNA binding (molecular function) GO:0008170 - N-methyltransferase activity (molecular function)
InterPro domains	IPR002941 - DNA methylase N-4/N-6 IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0041930.1 tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucumis melo var. makuwa]

7.1e-82

92.36

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL++PDHKFCL+E ADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LAT+GKLV+DPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

KAE8646077.1 hypothetical protein Csa_015923 [Cucumis sativus]

7.1e-82

92.36

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL++PDHKFCL+E ADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LAT+GKLV+DPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

XP_022149033.1 tRNA (guanine(10)-N2)-methyltransferase homolog [Momordica charantia]

3.8e-83

94.9

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL+NPDHKFCLMEMADYGLNNGLPPIAQR IFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LATAGKLVYDPFVGTGSILVAAAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLP PIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

XP_031744574.1 tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis sativus]

7.1e-82

92.36

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL++PDHKFCL+E ADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LAT+GKLV+DPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

XP_038888658.1 tRNA (guanine(10)-N2)-methyltransferase homolog [Benincasa hispida]

7.1e-82

92.36

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+V+L++PDHKFCLME AD+GLNNGLPPI+QRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LATAGKLVYDPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPI LLRADNNRPPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BG98 tRNA (Guanine(10)-N2)-methyltransferase homolog

3.5e-82

92.36

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL++PDHKFCL+E ADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LAT+GKLV+DPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

A0A5A7TF32 tRNA (Guanine(10)-N2)-methyltransferase-like protein

3.5e-82

92.36

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL++PDHKFCL+E ADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LAT+GKLV+DPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

A0A5D3D1Y2 tRNA (Guanine(10)-N2)-methyltransferase-like protein

3.5e-82

92.36

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL++PDHKFCL+E ADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LAT+GKLV+DPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

A0A6J1D5Q5 tRNA (Guanine(10)-N2)-methyltransferase homolog

1.8e-83

94.9

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL+NPDHKFCLMEMADYGLNNGLPPIAQR IFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LATAGKLVYDPFVGTGSILVAAAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLP PIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

A0A6J1ICG5 tRNA (Guanine(10)-N2)-methyltransferase homolog

1.0e-81

92.99

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNLRNPDHKF LME ADYGLNNGLPP+AQR IFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQ+LAT GKLVYDPFVGTGSILV AAH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLPPPIALLRADNN PPWRPGLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

SwissProt top hits

e value

%identity

Alignment

Q05B63 tRNA (guanine(10)-N2)-methyltransferase homolog

3.0e-30

40.85

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL+ P H F ++E  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN        +V+DPFVGTG +L+A+AH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG
        FGA   G DID   +          +  RGPD N+ +N +QYGL    + +L +D ++P WR G
Subjt:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG

Q5R962 tRNA (guanine(10)-N2)-methyltransferase homolog

3.0e-30

40.85

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL+ P H F ++E  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN        +V+DPFVGTG +L+A AH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG
        FGA   G DID   +          +  RGPD N+ +N +QYGL    + +L +D ++P WR G
Subjt:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG

Q7TNK6 tRNA (guanine(10)-N2)-methyltransferase homolog

7.8e-31

41.46

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL+ P H F ++E  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN +      LV+DPFVGTG +L+A+AH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG
        FGA   G DID   +          +  RGPD N+ +N +QYGL    + +L +D ++P WR G
Subjt:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG

Q7Z4G4 tRNA (guanine(10)-N2)-methyltransferase homolog

3.0e-30

40.85

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Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL+ P H F ++E  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN        +V+DPFVGTG +L+A AH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG
        FGA   G DID   +          +  RGPD N+ +N +QYGL    + +L +D ++P WR G
Subjt:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG

Q9CWH5 tRNA (guanine(10)-N2)-methyltransferase homolog

3.0e-30

40.85

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        G+VNL+ P H F ++E  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+M N +      LV+DPFVGTG +L+A+AH
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG
        FGA   G DID   +          +  RGPD N+ +N +QYGL    + +L +D ++P WR G
Subjt:  FGAMTMGADIDIRVI----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNRPPWRPG

Arabidopsis top hits

e value

%identity

Alignment

AT3G26410.1 methyltransferases;nucleic acid binding

3.1e-75

84.08

Show/hide

Query:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH
        GRVNL+NPDH F LMEM +   NNGL PI QRRIFFGREVG ADRKL+PT+QLKSRTYLGPTAMDAEMAFLMANQ+ AT+GKLVYDPFVGTGSILV+AA 
Subjt:  GRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATAGKLVYDPFVGTGSILVAAAH

Query:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL
        FGAMTMGADIDIRV+RDGRGPDCNVWSNFKQYGLP P+ALLR DNN PPWR GLKE+
Subjt:  FGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKEL

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGAAAAATCGCACTGTGGCTGATATCTTTGGCTCTGCGGGGAGCTCCCCTTCGTTCTCCAAAAGCTTTCATCGTCTGTTATCTGATAGAGAAACCACAAACATTCTGGC
CCTCTTATCGTTGTTAGAGGAGATTGAAGAAGAAACGAAGAAGAAGAAAGTAGAAACGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA
AGAAGAAGAAGAAGAAGAAACAAAAGAAGTTGAAGAAAGAAACGAAGAAGATGAAGAGGCAGCAGCGGCTAGGGTTGGATGTTGATGAGAAGCGTCGCCTTTTTATCGCC
TTTCGCCTTCGGGCGATCAAAGGGCTCGTCGCCTCGAGGTGCGCCTTGAAAACACTGAAGAAAACCATATTAGGTGTTGAGTCAGCTATCGGTCGAGTTAATTTAAGAAA
TCCAGATCATAAGTTCTGTCTGATGGAAATGGCTGATTATGGACTGAATAATGGTCTTCCACCAATTGCTCAAAGGAGGATTTTTTTTGGTCGGGAGGTTGGTGGTGCTG
ATAGGAAGCTTATACCAACATATCAGTTAAAAAGTCGCACTTATCTTGGGCCAACCGCCATGGACGCTGAAATGGCTTTCTTAATGGCCAACCAATCACTAGCGACAGCA
GGGAAACTTGTCTATGACCCATTTGTTGGCACTGGGAGTATTCTAGTTGCTGCAGCTCATTTTGGAGCAATGACAATGGGTGCAGACATTGACATCAGGGTGATTCGTGA
TGGTCGTGGCCCTGATTGTAATGTTTGGAGCAATTTCAAGCAGTATGGATTACCACCGCCAATTGCACTGTTAAGAGCAGATAATAATCGTCCTCCCTGGCGTCCAGGAT
TGAAAGAGTTGCTAACCAAGTGTAAAGATGTCTTGGGAATGGCCTTCTCACATCTTTCCGCCATGAATTCTCCATCTATTGATATGGTTGCATAA

mRNA sequence

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Protein sequence

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MKNRTVADIFGSAGSSPSFSKSFHRLLSDRETTNILALLSLLEEIEEETKKKKVETKKKKKKKKKKKKKKKKKKKKKKKKQKKLKKETKKMKRQQRLGLDVDEKRRLFIA
FRLRAIKGLVASRCALKTLKKTILGVESAIGRVNLRNPDHKFCLMEMADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQSLATA
GKLVYDPFVGTGSILVAAAHFGAMTMGADIDIRVIRDGRGPDCNVWSNFKQYGLPPPIALLRADNNRPPWRPGLKELLTKCKDVLGMAFSHLSAMNSPSIDMVA

Spg034913 (gene) of Sponge gourd (cylindrica) v1 genome