CuGenDBv2

Gene ID	Tan0001210
Organism	Trichosanthes anguina (Snake gourd v1)
Description	EF-hand domain-containing protein
Genome location	LG10:3286851..3290703
RNA-Seq Expression	Tan0001210
Synteny	Tan0001210
Gene Ontology terms	GO:0005509 - calcium ion binding (molecular function) GO:0048306 - calcium-dependent protein binding (molecular function)
InterPro domains	IPR002048 - EF-hand domain IPR011992 - EF-hand domain pair IPR018247 - EF-Hand 1, calcium-binding site

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6582540.1 hypothetical protein SDJN03_22542, partial [Cucurbita argyrosperma subsp. sororia]

9.1e-57

75.3

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        M +ALGRFGGKEWRDEQLR ISDRIF+ I ++  KDKLSFEDLYIATLLVYNDINK LP  HLDPPT+ER +ELI+++D + D++I R+EFL FILSLTS
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        D FIS+S RLILTLV+APTVAVVTK+STEG+P VG LVQKLP+SAYAFLVTLAA +FQNSQ+ +LK
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

XP_022146952.1 uncharacterized protein LOC111016024 [Momordica charantia]

7.4e-59

77.78

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        MGQ L + GGK+WRD Q+RKISD+IFNKIKN S K  LSFEDLYIATLLVYN+INK LPGPH DPP+KERV+ELI++SD + D+EIDREEF+ FIL LTS
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQ
        DT I+VSQ L+LTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPAS YA L+TLAAL FQN+ Q
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQ

XP_023528258.1 uncharacterized protein LOC111791228 [Cucurbita pepo subsp. pepo]

9.1e-57

75.3

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        M +ALGRFGGKEWRDEQLR ISDRIF+ I ++  KDKLSFEDLYIATLLVYNDINK LP  HLDPPT+ER +ELI+++D + D++I R+EFL FILSLTS
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        D FIS+S RLILTLV+APTVAVVTK+STEG+P VG LVQKLP+SAYAFLVTLAA +FQNSQ+ +LK
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

XP_038876686.1 uncharacterized protein LOC120069050 [Benincasa hispida]

5.1e-76

92.17

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        MGQ+LGRFGGKEWRDEQLRKIS++IF+K KNQSH+DKLSFEDLYIATLLVYNDINK +PGPHLDPPTKERVKELIQDSDRD+DREIDREEFLDFILSLTS
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        DTF+SVSQRLILTLVVAPTVAVVTKKSTEGVPG+GK VQKLPASAYAFLVTLAALMFQNSQQ LLK
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

XP_038892374.1 uncharacterized protein LOC120081499 [Benincasa hispida]

2.6e-59

78.36

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPT----KERVKELIQDSDRDDDREIDREEFLDFIL
        MGQAL  FGGKEWRD+QLRKI+DRIF+K + Q+H DKL  EDLYIATLLVYN+INKL+PGPH DPPT    KERVKELIQ SD + D+ IDR EFL FIL
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPT----KERVKELIQDSDRDDDREIDREEFLDFIL

Query:  SLTSDTFISVSQRL-ILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        SLTS+TF SVSQRL ILTLVVAPTVAVVTKKSTE +PGVGKLVQKLP SAYAFLVTLAAL+FQ SQQ LL+
Subjt:  SLTSDTFISVSQRL-ILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

TrEMBL top hits

e value

%identity

Alignment

A0A1S3AVI1 uncharacterized protein LOC103483388

2.3e-53

68.07

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        MGQAL RF G++W+++QL  IS++IF++     HK+KLSFEDLYIATLLV+NDINK LPGPH+DPPTKERV EL++ SD+++++ IDR+EFL FI  LT 
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        ++  SVSQRLI+TLVVAPT+AVVTK+STEG+PG+GK+VQK+P+SAYA LVTLAAL+FQNS Q LLK
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

A0A5A7U485 EF-hand domain-containing protein

4.5e-54

68.67

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        MGQAL RFGG++W+++QL  IS++IF++     HK+KLSFEDLYIATLLV+NDINK LPGPH+DPPTKERV EL++ SD ++++ IDR+EFL FI  LT 
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        ++  SVSQRLI+TLVVAPT+AVVTK+STEG+PG+GK+VQK+P+SAYA LVTLAAL+FQNS Q LLK
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

A0A6J1D109 uncharacterized protein LOC111016024

3.6e-59

77.78

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        MGQ L + GGK+WRD Q+RKISD+IFNKIKN S K  LSFEDLYIATLLVYN+INK LPGPH DPP+KERV+ELI++SD + D+EIDREEF+ FIL LTS
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQ
        DT I+VSQ L+LTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPAS YA L+TLAAL FQN+ Q
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQ

A0A6J1EC16 uncharacterized protein LOC111431834

5.7e-57

74.7

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        M +ALGRFGGKEWRDEQLR ISDRIF+ I ++  KDKLSFEDLYIATLLVYNDINK LP  HLDPPT+ER +ELI+++D + D++I R+EFL FILSLTS
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        D FIS+S RLILTLV+APTVAVVTK+STEG+P +G LVQKLP+SAYAFLVTLAA +FQNSQ+ +LK
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

A0A6J1ITL1 uncharacterized protein LOC111479803

1.3e-56

74.1

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        M +ALGRFGGKEWRDEQLR ISDRIF+ I ++  KDKLSFEDLYIATLLVYNDINK LP  HLDPPT+ER ++L++++D ++D++I R+EFL FILSLTS
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK
        D FIS+S RLILTLV+APTVAVVTK+STEG+P VG LVQKLP+SAYAFLVTLAA +FQNSQ+ +LK
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G64850.1 Calcium-binding EF hand family protein

1.7e-45

58.28

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        MGQ   +  GKEWR +Q++ I DR+F++ K Q+ +  L+FE+LYIA LLVYNDINK LPGPH DPP+K+ V  ++ D D + D E+DREEF+ FI  +T+
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQA
        +TF  VSQ LI+ L+VAPTVA+ TKK+TEGVPGVGK+VQKLP S YA LVTL A+M+ NS  +
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQA

AT4G37445.1 BEST Arabidopsis thaliana protein match is: Calcium-binding EF hand family protein (TAIR:AT1G64850.1)

2.0e-06

24.36

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Query:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS
        MG ++G  G      + +  +   ++   K  + K   +F+D ++A L ++N+ N  LPG H D PT +++K          D E  ++ F++F+     
Subjt:  MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTS

Query:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALM
         +   +    ++T +V+P  A+  K++ E VP + KL++ +P   +   VT+ A++
Subjt:  DTFISVSQRLILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALM

Sequences

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CDS sequence

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ATGGGACAAGCCTTGGGGAGATTTGGAGGCAAGGAATGGAGGGACGAACAATTGAGGAAGATATCAGACAGGATATTTAATAAGATTAAAAATCAATCACATAAGGATAA
GCTGTCATTTGAAGATCTGTACATCGCTACGTTACTTGTGTACAATGATATTAATAAGCTGTTGCCCGGCCCACATCTTGATCCGCCCACAAAAGAACGTGTCAAAGAAC
TCATACAGGACAGCGATAGGGACGACGACCGCGAAATCGACCGTGAAGAATTTTTGGATTTTATATTGAGCTTGACATCTGACACGTTCATATCAGTGAGCCAGAGGTTG
ATCCTCACTTTGGTGGTTGCACCAACAGTCGCAGTGGTGACAAAGAAAAGTACTGAAGGCGTTCCTGGTGTTGGAAAACTGGTGCAAAAACTGCCCGCTTCAGCATATGC
TTTCCTCGTCACTCTTGCTGCTCTGATGTTTCAAAACTCTCAACAGGCGCTTCTTAAATGA

mRNA sequence

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CCGCTTGATTATTCTATTTTCTCCGTATTTTGGGACAAAACCAAATGTATAACTTGCATTCAAGGAAACCGAAGGTCGCTTTATTCCAAGTTTCCTTTCGAACAGGAAAA
GGAAATGTGTGGCAGAAGCAGAATGGTGTATGAATTTTATCTTTTTCTAATACAAAACCTCTGGAAATGATAAATTTCGATATCGTTGCTGCTACGGCGGACATCCTCAA
TCTTCCTTTGCACGAAGAATCGATTTAAATCACAGTCGGGGGCTGCTTTGCGAGGCCTGTGATATTCAATCGCCGTCGCTGCCGTCTAGCATGGGACAAGCCTTGGGGAG
ATTTGGAGGCAAGGAATGGAGGGACGAACAATTGAGGAAGATATCAGACAGGATATTTAATAAGATTAAAAATCAATCACATAAGGATAAGCTGTCATTTGAAGATCTGT
ACATCGCTACGTTACTTGTGTACAATGATATTAATAAGCTGTTGCCCGGCCCACATCTTGATCCGCCCACAAAAGAACGTGTCAAAGAACTCATACAGGACAGCGATAGG
GACGACGACCGCGAAATCGACCGTGAAGAATTTTTGGATTTTATATTGAGCTTGACATCTGACACGTTCATATCAGTGAGCCAGAGGTTGATCCTCACTTTGGTGGTTGC
ACCAACAGTCGCAGTGGTGACAAAGAAAAGTACTGAAGGCGTTCCTGGTGTTGGAAAACTGGTGCAAAAACTGCCCGCTTCAGCATATGCTTTCCTCGTCACTCTTGCTG
CTCTGATGTTTCAAAACTCTCAACAGGCGCTTCTTAAATGATAAGTTGAGACTCTGTTTCATTCCTTTGTTATTTGCATATATATCGTTATGAACAAATCGACACTCGAA
CTTTGAATTTACCATTTGAAGCCTTTGTTTGTTCTAGTTTGTTTCTATGGCTGTTCATCCTTTTCTTTCCCTTCATGGAGGTCTCTCAGTCTCCACTGATGATTTAGTTT
TTGATGCGTTGTTTGAAGCCTTGTTTGTTGTATTTTGTTTCTATGGCTGTTCATCCTTTTCTTTCCCTTCATGAAGGTCTCTCCATTCAAACTGTTTGGATCGTCCATTC
TCCTCCGTATCAAGAGAGACGAGAGTTTTGTGGCGGGCTAGGGTGTGTGCTATTTTGTGGGGTTTTGGGTGAGTGAAACAACAAAGTCTTTCGTGGGCTTGAGAGATCTC
CTGGTAATGTGTGGTTACTTATTACTTCCATGTTTCTCTTTGGGTTGTAGTGATAAACTTTTTTGTAATTATACACTAGATCTTATTTTAATTGACTAAAGGTCTTTTTG
TA

Protein sequence

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MGQALGRFGGKEWRDEQLRKISDRIFNKIKNQSHKDKLSFEDLYIATLLVYNDINKLLPGPHLDPPTKERVKELIQDSDRDDDREIDREEFLDFILSLTSDTFISVSQRL
ILTLVVAPTVAVVTKKSTEGVPGVGKLVQKLPASAYAFLVTLAALMFQNSQQALLK

Tan0001210 (gene) of Snake gourd v1 genome