CuGenDBv2

Gene ID	Tan0013066
Organism	Trichosanthes anguina (Snake gourd v1)
Description	tRNA(Ile)-2-lysyl-cytidine synthase
Genome location	LG07:4713251..4715852
RNA-Seq Expression	Tan0013066
Synteny	Tan0013066
Gene Ontology terms	GO:0006400 - tRNA modification (biological process) GO:0009658 - chloroplast organization (biological process) GO:0009793 - embryo development ending in seed dormancy (biological process) GO:0010098 - suspensor development (biological process) GO:0005524 - ATP binding (molecular function) GO:0016879 - ligase activity, forming carbon-nitrogen bonds (molecular function)
InterPro domains	IPR011063 - tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal IPR012094 - tRNA(Ile)-lysidine synthase IPR014729 - Rossmann-like alpha/beta/alpha sandwich fold

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6587963.1 hypothetical protein SDJN03_16528, partial [Cucurbita argyrosperma subsp. sororia]

3.9e-36

91.57

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWKMD LNATGKSS+FIDGLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

KAG7021853.1 tilS, partial [Cucurbita argyrosperma subsp. argyrosperma]

3.9e-36

91.57

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWKMD LNATGKSS+FIDGLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

XP_022933762.1 uncharacterized protein LOC111441079 isoform X1 [Cucurbita moschata]

3.9e-36

91.57

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWKMD LNATGKSS+FIDGLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

XP_022933764.1 uncharacterized protein LOC111441079 isoform X2 [Cucurbita moschata]

3.9e-36

91.57

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWKMD LNATGKSS+FIDGLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

XP_023529428.1 uncharacterized protein LOC111792288 isoform X1 [Cucurbita pepo subsp. pepo]

3.9e-36

91.57

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWKMD LNATGKSS+FIDGLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

TrEMBL top hits

e value

%identity

Alignment

A0A5N6QHE1 tRNA(Ile)-2-lysyl-cytidine synthase

7.4e-33

80.72

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVLTA WK +GLNA G+SS FIDG+LAI+VDHGLRAES+DEAN+V NRV+QMGI+CEI  CDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

A0A6J1CVA6 tRNA(Ile)-2-lysyl-cytidine synthase

1.2e-35

87.95

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALC LTA WKMDGLNATGK S+FIDGLLAIVVDHGLRAESRDEA VVWNRVT +GI+CEI SCDW DGRPKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

A0A6J1F0N5 tRNA(Ile)-2-lysyl-cytidine synthase

1.9e-36

91.57

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWKMD LNATGKSS+FIDGLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

A0A6J1F0R0 tRNA(Ile)-2-lysyl-cytidine synthase

1.9e-36

91.57

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWKMD LNATGKSS+FIDGLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

A0A6J1I6Q1 tRNA(Ile)-2-lysyl-cytidine synthase

1.8e-34

87.95

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVL AGWK D LN TGKSS+FI GLLAIVVDHGLRAESRDEANVV NRVTQMGI+CEI SCDWLDG+PKQGHLQEAARDM
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT3G24560.2 Adenine nucleotide alpha hydrolases-like superfamily protein

1.1e-31

72.29

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Query:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM
        MALCVLTA WK +GL+   K+  FIDGL+AIVVDHGLR ES+DEA +V +RV+QMGI+CEI SCDW+DGRPK GHLQEAAR+M
Subjt:  MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDM

Sequences

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CDS sequence

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ATGGCTCTCTGTGTTCTTACAGCGGGCTGGAAAATGGATGGCTTGAATGCCACTGGTAAAAGTAGCTCGTTTATCGATGGGCTCCTGGCAATAGTCGTGGATCATGGGCT
TCGTGCTGAGAGCAGAGACGAGGCAAATGTTGTCTGGAATCGAGTTACTCAAATGGGTATCAAATGCGAGATTGTGTCTTGTGATTGGTTAGACGGTAGACCAAAGCAGG
GCCACTTGCAAGAAGCAGCTCGTGATATGAGCGGGAGAGAACGTGAGTGGAATAGGAAAGTAGAATAA

mRNA sequence

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Protein sequence

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MALCVLTAGWKMDGLNATGKSSSFIDGLLAIVVDHGLRAESRDEANVVWNRVTQMGIKCEIVSCDWLDGRPKQGHLQEAARDMSGREREWNRKVE

Tan0013066 (gene) of Snake gourd v1 genome