| GenBank top hits | e value | %identity | Alignment |
|---|
| KAG7024282.1 Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma] | 2.3e-111 | 72.24 | Show/hide |
Query: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
+SLSLLFTL LI KLRK + ++ PPSPPSLP+IGHLHLLK+PFH+VLQ+LS+K+GPIL LSIGSRP+VV+SSPTA++ECFTKNDI ANRPRLLSG Y+N
Subjt: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
Query: YNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
YNYT + ASY HHWRN+ T ELLSN L+TYLNIRVQELKL VKNLYG TG+S DFVR+EM+S+LNELSFNTVMRM+SGKRYFGV+VEDVEEAREF
Subjt: YNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
Query: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
R+IMKELLQLSG+ +FD+QGLEKRM KAS RAD F+QNLID +R++R+ R P++K G KTMIQSLLS QES P YYSDDIIKG VL L
Subjt: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
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| XP_022936846.1 isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] | 5.4e-116 | 74.25 | Show/hide |
Query: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
+SLSLLFTL LI KLRK + ++ PPSPPSLP+IGHLHLLK+PFH+VLQ+LS K+GPIL LSIGSRP+VV+SSPTA++ECFTKNDI ANRPRLLSGKY+N
Subjt: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
Query: YNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
YNYT + ASY HHWRN + TTELLSNH LN YLNIRVQELKLWVKNLYGETG+S DFVR+EM+S+LNELSFNTVMRM+SGKRYFGV+VEDVEEAREF
Subjt: YNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
Query: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
R+IMKELL LSG+ +FD+QGLEKRM KASGRAD F+QNLID +R++RA R P++K G KTMIQSLLS QES P YYSDDIIKG VL L
Subjt: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
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| XP_022977005.1 isoflavone 3'-hydroxylase-like [Cucurbita maxima] | 8.0e-112 | 71.7 | Show/hide |
Query: MEAETGWSSCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLA
MEA T +C +SLSLL TLILI KLRK + + PPSPPSLP+IGHLHLLK+PFH+VLQ+LS+K+GPIL LSIGSRP+VV+SSPTA++ECFTKNDI LA
Subjt: MEAETGWSSCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLA
Query: NRPRLLSGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG
NRPRLL+GKY+NYNYT + ASY HHWRN + TTELLSNH LN YLNIRVQELKL VKNLYG TG+S DFVR+EM+S+LNELSFN VMRM+SGKRYFG
Subjt: NRPRLLSGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG
Query: VEVEDVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSD
V+VEDVEEAREFR+IMKELLQL G+ +FD+QGLEKRM +ASGRAD+F+QNLID +R +RA R P++K+G KTMIQSLLSLQES P +YSD
Subjt: VEVEDVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSD
Query: DIIKGQVLVNL
DIIKG VL L
Subjt: DIIKGQVLVNL
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| XP_023535658.1 isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo] | 1.7e-117 | 75.25 | Show/hide |
Query: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
+SLSLLFTLILI KLRK + +K PPSPPSLP+IGHLHLLK+PFH+VLQ+LS+K+GPIL LSIGSRP+VV+SSPTA++ECFTKNDI ANRPRLLSGKY+N
Subjt: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
Query: YNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
YNYT + ASY HHWRN + TTELLSNH LNTYLNIRVQELKLWVKNLYG TG+S DFVR+EM+S+LNELSFNTVMRM+SGKRYFGV+VEDVEEAREF
Subjt: YNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
Query: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
R+IMKELLQLSG+ +FD+QGLEKRM KASGRAD F+QNLID +R++RA P++K G KTMIQSLLSLQES P YYSDDIIKG VL L
Subjt: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
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| XP_038896686.1 cytochrome P450 81Q32-like [Benincasa hispida] | 5.0e-114 | 73.68 | Show/hide |
Query: WSSCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLL
W FLISL LFT IL+SKLRKINHKKLPPSPPSLPVIGHLHLL+ P H+VL NLS+KYGPIL LSIGSRPVVVVSSPTA++ECFTKNDIV ANRPRLL
Subjt: WSSCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLL
Query: SGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDV
SGKYINYNYTA+G A Y HWRN + TTELLSNH LNTYLNIRVQELKLW+KNLY + +FV VEMKS+L ELSFN VMRM+SGKRYFGVEV DV
Subjt: SGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDV
Query: EEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
EEA EFR++ KELL+LSG+ VFDFQGL KRM KASGRAD F+Q LIDEQR++R R P+ K GK+MIQ+LLSLQES P YYSDDIIKG V
Subjt: EEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
Query: LVNL
L L
Subjt: LVNL
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| TrEMBL top hits | e value | %identity | Alignment |
|---|
| A0A5A7VB12 Cytochrome P450 81E8-like | 9.0e-109 | 71.38 | Show/hide |
Query: FLISLSLLFTLILISKLRKI-NHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGK
F +SL L+ TLIL+SKLRK+ N KKLPP+PPSLP+IGHLHLLK PFH+VL NLS+KYGPIL L+IGSRPV+VVSSPTA++ECFTKNDIV ANRPRL+SGK
Subjt: FLISLSLLFTLILISKLRKI-NHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGK
Query: YINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEA
YINYNYTA+G A Y HWRN + TTELLSNH LN YLNIRV+ELKLWVKN + G DFV V+MKS+L ELSFNTVMRMISGKRYFGVEVEDVEEA
Subjt: YINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEA
Query: REFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPD--QKQGKT-MIQSLLSLQESHPHYYSDDIIKGQV
EFR+IMKELL+LSG+ VFD +G++KRM KASGRAD F+Q LIDE+R+KR R P+ QKQ KT MI++LLSLQES P YYSDDIIKG V
Subjt: REFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPD--QKQGKT-MIQSLLSLQESHPHYYSDDIIKGQV
Query: LVNL
L L
Subjt: LVNL
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| A0A6J1C5W0 isoflavone 3'-hydroxylase-like | 3.3e-111 | 68.99 | Show/hide |
Query: MEAETGWSSCFLISLSLLF--TLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIV
ME+ET W S FLI L+LL L++ISKLRK++HK LPPSPPS PVIGHLHLL+ PFH+VL NLS KYGPIL L+IGSRPVVV++SPTA++ECFTKND+V
Subjt: MEAETGWSSCFLISLSLLF--TLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIV
Query: LANRPRLLSGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLY----GETGKSRDFVRVEMKSRLNELSFNTVMRMIS
ANRPRLLSGKYINYNYTA+G A Y HWRN L TTELLSNH LNT+LN+RVQELKLWVKNLY +FVRVEMKSRLN+LSFNT+MRM+S
Subjt: LANRPRLLSGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLY----GETGKSRDFVRVEMKSRLNELSFNTVMRMIS
Query: GKRYFGVEVEDVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHP
GKRY+G EVEDVEEAREFR+IMKELL+LSG+ VFD+QGLEKRM KA+ R+D+F+Q LID QR++R+ R D+ GK MI LLSLQES P
Subjt: GKRYFGVEVEDVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHP
Query: HYYSDDIIKGQVLVNL
YYSDDIIKG VL L
Subjt: HYYSDDIIKGQVLVNL
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| A0A6J1F8L9 isoflavone 3'-hydroxylase-like isoform X1 | 2.6e-116 | 74.25 | Show/hide |
Query: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
+SLSLLFTL LI KLRK + ++ PPSPPSLP+IGHLHLLK+PFH+VLQ+LS K+GPIL LSIGSRP+VV+SSPTA++ECFTKNDI ANRPRLLSGKY+N
Subjt: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
Query: YNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
YNYT + ASY HHWRN + TTELLSNH LN YLNIRVQELKLWVKNLYGETG+S DFVR+EM+S+LNELSFNTVMRM+SGKRYFGV+VEDVEEAREF
Subjt: YNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
Query: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
R+IMKELL LSG+ +FD+QGLEKRM KASGRAD F+QNLID +R++RA R P++K G KTMIQSLLS QES P YYSDDIIKG VL L
Subjt: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
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| A0A6J1F9Z0 isoflavone 3'-hydroxylase-like | 1.6e-110 | 72.54 | Show/hide |
Query: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
+SLSLLFTL LI KLRK + ++ PPSPPSLP+IGHLHLLK+PFH+VLQ+LS K+GPIL LSIGSRP+VV+SSPTA++ECFTKNDI ANRPRLLSGKY+N
Subjt: ISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYIN
Query: YNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
YNYT + ASY HHWRN+ T ELLSN L+TYLNIRVQELKL VKNLYGETG+S DFVR+EM+S+LNELSFNTVMRM+SGKRYFGV+VEDVEEAREF
Subjt: YNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAREF
Query: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQV
++IMKELL LSG+ +FD+QGLEKRM KASGRAD F+Q LID +R++RA R PD+K G KTMIQSLLS QES P YYSDDII V
Subjt: RKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSDDIIKGQV
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| A0A6J1IL26 isoflavone 3'-hydroxylase-like | 3.9e-112 | 71.7 | Show/hide |
Query: MEAETGWSSCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLA
MEA T +C +SLSLL TLILI KLRK + + PPSPPSLP+IGHLHLLK+PFH+VLQ+LS+K+GPIL LSIGSRP+VV+SSPTA++ECFTKNDI LA
Subjt: MEAETGWSSCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLA
Query: NRPRLLSGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG
NRPRLL+GKY+NYNYT + ASY HHWRN + TTELLSNH LN YLNIRVQELKL VKNLYG TG+S DFVR+EM+S+LNELSFN VMRM+SGKRYFG
Subjt: NRPRLLSGKYINYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG
Query: VEVEDVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSD
V+VEDVEEAREFR+IMKELLQL G+ +FD+QGLEKRM +ASGRAD+F+QNLID +R +RA R P++K+G KTMIQSLLSLQES P +YSD
Subjt: VEVEDVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQG-KTMIQSLLSLQESHPHYYSD
Query: DIIKGQVLVNL
DIIKG VL L
Subjt: DIIKGQVLVNL
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| SwissProt top hits | e value | %identity | Alignment |
|---|
| P93147 Isoflavone 2'-hydroxylase | 1.9e-68 | 46.73 | Show/hide |
Query: SCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSG
S F ++L +F ++ +R K LPP PPSLP+IG+LH LKRP H+ + LS KYG + L GSR VVVVSS + ++CFTKND+VLANRPR LSG
Subjt: SCFLISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSG
Query: KYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVEDV
KYI YNYT +GS SY HWRNL ++LSNH +N++ IR E + + L ++ S +F +E+ SRL +++FN +MRMISGKRY+G + D+
Subjt: KYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVEDV
Query: EEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
+EA +FR ++ ELLQLSG+ DF+ LEKR+ SG+ DAF++ LI+E R K+ ++ TMI LL+LQ+S P YY+D IIKG
Subjt: EEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
Query: LVNLIS
L L++
Subjt: LVNLIS
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| Q6WNQ8 Cytochrome P450 81E8 | 1.4e-63 | 47.4 | Show/hide |
Query: FLISLSL-LFTLILISKL---RKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLL
F +SL + LF LI+ K+ K LPP P LP+IG+LH LK+P H LS KYG I L GSR VVVVSS T +ECFTKNDIVLANRP L
Subjt: FLISLSL-LFTLILISKL---RKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLL
Query: SGKYINYNYTAMGSASYDHHWRNLP---TTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVE
+GKYI YN T + + Y HWRNL + E+LS+H LN++L IR E+ ++ L ++ F VE++ +E++FNT+MRM+SGKRY+G +V
Subjt: SGKYINYNYTAMGSASYDHHWRNLP---TTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVE
Query: DVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKG
DVEEAR FR I+KE++ L G+ FDF GLEKR+ K S R DAF+Q LIDE R ++ TMI LL+ Q+S P YY+D IIKG
Subjt: DVEEAREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKG
Query: QVLVNLIS
++V L++
Subjt: QVLVNLIS
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| Q6WNQ9 Isoflavone 3'-hydroxylase (Fragment) | 3.1e-66 | 46.98 | Show/hide |
Query: LISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYI
L+SLS + T+ ++ K+ K LPP PP++P+IG+LH LK P H+ LS YG I L GSR VVVVSSP+ ECFTKNDI+LANRPR L+GKYI
Subjt: LISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYI
Query: NYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGE-TGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVEDVEE
YNYT +GSASY HWRNL T ++LSN+ LN++L +R E ++ L + + F +VE++ RL E++FN +MRMISGKRY+G +V DVEE
Subjt: NYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGE-TGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVEDVEE
Query: AREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
A++FR+I+ E++ L G+ V D LEKR + + R++AF++ LI+E R+ G TMI LL L ES P YYSD +IKG +
Subjt: AREFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
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| Q6WNR0 Isoflavone 2'-hydroxylase | 5.0e-64 | 46.2 | Show/hide |
Query: LISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYI
L LS+ F + L+ + RK K LPP P SLP+IG+LH LKRP ++ + L+ KYG ++ L GSR VVVVSS + +ECFTKND+VLANRPR LSGKYI
Subjt: LISLSLLFTLILISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKYI
Query: NYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVEDVEEA
YNYT +GS SY HWRNL + ++LSNH +N + IR E + +K L ++ S F VE+ R +++FN +MRMISGKRY+G ++ +V+EA
Subjt: NYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFG--VEVEDVEEA
Query: REFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLVN
+FR ++ ELLQLSG+ DF+ LEKR+ + + D F+ L+ EQR K+ ++ TMI LL++QES P YY+D IIKG L
Subjt: REFRKIMKELLQLSGS-----------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLVN
Query: LIS
L++
Subjt: LIS
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| W8JMU7 Cytochrome P450 81Q32 | 1.0e-61 | 47.02 | Show/hide |
Query: LISLSLLFTLILISKLRKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKY
++ LS+ L +S+ R + LPPSP +LPVIGHLHL+ + H+ L +LS KYG + L +G+R V+VVSSP A +ECFTKNDIV ANRP + GKY
Subjt: LISLSLLFTLILISKLRKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSGKY
Query: INYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAR
I YNYT M + Y HWRN L E+ S LN +L+IR E+K + +LY +G +DF +VEMKS+L+ELSFN MRM++GKRYFG +V D +EA+
Subjt: INYNYTAMGSASYDHHWRN---LPTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEEAR
Query: EFRKIMKELLQLSGSV-----------FDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
FR ++ E+ + +G+ DF+ EK++SK S DAF+Q LI E R K TMI LLSLQES P YY+D IIKG ++V L
Subjt: EFRKIMKELLQLSGSV-----------FDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
Query: IS
++
Subjt: IS
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| Arabidopsis top hits | e value | %identity | Alignment |
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| AT2G23220.1 cytochrome P450, family 81, subfamily D, polypeptide 6 | 2.1e-54 | 41.83 | Show/hide |
Query: FLISLSLLFTLILISKL---RKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYG--PILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPR
+ + LSL F L + KL ++++ LPPSP LP+IGHLHLLK P H+ + S G PI L +G+R VVVSS + +ECFTKNDIV ANRP
Subjt: FLISLSLLFTLILISKL---RKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYG--PILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPR
Query: LLSGKYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVE
L+ GK+I YN T M SA Y HWRNL T E+ ++H LN +L++R E++ + L F +VEM+ L EL+ N V RM++GKR++G E
Subjt: LLSGKYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVE
Query: DVEEAREFRKIMKELLQLSGS---------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
E A++ R +M E++ +G+ + F EKR+ + R D F+Q+L+DE+R D+++G TMI LLSLQES P YY+D +KG +
Subjt: DVEEAREFRKIMKELLQLSGS---------VFDFQGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQV
Query: LVNLIS
+V +I+
Subjt: LVNLIS
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| AT4G37320.1 cytochrome P450, family 81, subfamily D, polypeptide 5 | 5.3e-53 | 39.8 | Show/hide |
Query: LISLSLLFTLILISKLRKINHKK--LPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYG--PILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLL
++ LS LF I I L +++K LPPSP LPVIGHLHLLK+P H+ ++S G PI +L +G+R V V+SS + +ECFTKND+VLANRP ++
Subjt: LISLSLLFTLILISKLRKINHKK--LPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYG--PILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLL
Query: SGKYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDV
K++ YN+T M +ASY HWRNL E+ S+H ++T+ +IR E++ + +L ++ FV VE+KS L L+FN ++ M++GKRY+G ED
Subjt: SGKYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDV
Query: EEAREFRKIMKELL--QLSGSVFDF-------QGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLV
+EA+ R+++ E++ SG++ D+ E + R D +Q L+DE+R ++++G+T+I LLS QE+ P YY+D IIKG +L
Subjt: EEAREFRKIMKELL--QLSGSVFDF-------QGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLV
Query: NLIS
+++
Subjt: NLIS
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| AT4G37330.1 cytochrome P450, family 81, subfamily D, polypeptide 4 | 3.1e-53 | 41.39 | Show/hide |
Query: LISLSLLFTLILISKLRKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYG--PILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSG
++S LFTL + R LPPSP SLPVIGHLHLLK P H+ +LS G P+ +L +G+R V V+SS + +ECFTKND+VLANRP+
Subjt: LISLSLLFTLILISKLRKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKYG--PILYLSIGSRPVVVVSSPTAIKECFTKNDIVLANRPRLLSG
Query: KYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEE
K++ YN T + SASY HWRNL E+ S H LN++L IR E++ + +L ++ FV VEMK+ L L+ NT +RM++GKRYFG ED ++
Subjt: KYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVEVEDVEE
Query: AREFRKIMKELLQL--SGSVFDFQGL-------EKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
A+ + ++ E + +G+ D+ + EKR+ R D F+Q L+DE+R ++++G+TMI LL+LQ+ P YY+D IIKG +L +
Subjt: AREFRKIMKELLQL--SGSVFDFQGL-------EKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIKGQVLVNL
Query: IS
I+
Subjt: IS
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| AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8 | 7.1e-58 | 42.81 | Show/hide |
Query: MEAETGWSSCFLISLSLLFTLILISKLRKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKY--GPILYLSIGSRPVVVVSSPTAIKECFTKNDI
ME +T S + LSL++ LI KL++ LPPSP SLPVIGHL LLK P H+ +LS PI L +G+R V V SS + +ECFTKND+
Subjt: MEAETGWSSCFLISLSLLFTLILISKLRKINHKKLPPSPP-SLPVIGHLHLLKRPFHQVLQNLSHKY--GPILYLSIGSRPVVVVSSPTAIKECFTKNDI
Query: VLANRPRLLSGKYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKR
VLANRP + K++ Y+YT M +ASY HWRNL + E+ SNH LN++L+IR E++ V L S++FV+V+MKS L++L+FN ++RM++GKR
Subjt: VLANRPRLLSGKYINYNYTAMGSASYDHHWRNL---PTTELLSNHCLNTYLNIRVQELKLWVKNLYGETGKSRDFVRVEMKSRLNELSFNTVMRMISGKR
Query: YFGVEVEDVEEAREFRKIMKELLQL--SGSVFDF-------QGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSD
Y+G VED EA+ R+++ +++ +G+ D+ E R+ K +GR D F+Q L+DE+R+ + ++G TMI LL+LQES P Y++D
Subjt: YFGVEVEDVEEAREFRKIMKELLQL--SGSVFDF-------QGLEKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSD
Query: DIIKGQVLVNLIS
IIKG +L +++
Subjt: DIIKGQVLVNLIS
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| AT4G37430.1 cytochrome P450, family 91, subfamily A, polypeptide 2 | 4.8e-54 | 40.13 | Show/hide |
Query: FLISLSLLFTLI----LISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFT-KNDIVLANRPRL
+ I L LLF +I L SK ++ N LPP PPS P +GHLHL+K P H++LQ S++YGPI L GSR VVV++SP+ +E FT +NDIVL++RP
Subjt: FLISLSLLFTLI----LISKLRKINHKKLPPSPPSLPVIGHLHLLKRPFHQVLQNLSHKYGPILYLSIGSRPVVVVSSPTAIKECFT-KNDIVLANRPRL
Query: LSGKYINYNYTAMGSASYDHHWRNLP---TTELLSNHCLNTYLNIRVQELKLWVKNL--YGET-GKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVE
L+ KY+ YN+T +G+A Y HWRNL + E+LS+H L + +IR E+ + L Y +T +S DF +E++ L++L+FN ++RM++GKRY+G +
Subjt: LSGKYINYNYTAMGSASYDHHWRNLP---TTELLSNHCLNTYLNIRVQELKLWVKNL--YGET-GKSRDFVRVEMKSRLNELSFNTVMRMISGKRYFGVE
Query: VEDVEEAREFRKIMKELLQLSGSVFDFQGL----------EKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIK
V + EEA F+K++ ++ SG+ L EK + D +Q L+DE R+ K+G TM+ L+SLQ+ P YY+D IIK
Subjt: VEDVEEAREFRKIMKELLQLSGSVFDFQGL----------EKRMSKASGRADAFVQNLIDEQRQKRALRLPDQKQGKTMIQSLLSLQESHPHYYSDDIIK
Query: GQVLVNLIS
G ++ +++
Subjt: GQVLVNLIS
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