CuGenDBv2

Gene ID	Tan0021037
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Allene oxide synthase, chloroplastic
Genome location	LG05:3378202..3380883
RNA-Seq Expression	Tan0021037
Synteny	Tan0021037
Gene Ontology terms	GO:0004497 - monooxygenase activity (molecular function) GO:0005506 - iron ion binding (molecular function) GO:0016705 - oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen (molecular function) GO:0020037 - heme binding (molecular function)
InterPro domains	IPR036396 - Cytochrome P450 superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7029361.1 Allene oxide synthase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.5e-20

42.77

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        + D+RV+ +     F   F   + +   + TG ++  T  +   R LS    S      +K    F+L              F ELF+TLEKDLA  GRA
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        EYNAP EQA FNFLARSLF  NP DTKL +DAPKLI+KWVLF+L    R   P+   ELL    RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

XP_022962362.1 allene oxide synthase 1, chloroplastic-like [Cucurbita moschata]

1.5e-20

42.77

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        + D+RV+ +     F   F   + +   + TG ++  T  +   R LS    S      +K    F+L              F ELF+TLEKDLA  GRA
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        EYNAP EQA FNFLARSLF  NP DTKL +DAPKLI+KWVLF+L    R   P+   ELL    RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

XP_022996510.1 allene oxide synthase 1, chloroplastic-like [Cucurbita maxima]

2.0e-20

42.17

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        + D+RV+ +     F   F   + +   + TG ++  T  +   R LS    S      +K    F+L              F ELF+TLEKDLA  GRA
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        EYNAP EQA FNFLARSLF  NP DTKL +DAPKLI+KW+LF+L    R   P+   ELL    RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

XP_023547133.1 allene oxide synthase 1, chloroplastic-like [Cucurbita pepo subsp. pepo]

1.5e-20

42.77

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        + D+RV+ +     F   F   + +   + TG ++  T  +   R LS    S      +K    F+L              F ELF+TLEKDLA  GRA
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        EYNAP EQA FNFLARSLF  NP DTKL +DAPKLI+KWVLF+L    R   P+   ELL    RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

XP_038885277.1 allene oxide synthase 1, chloroplastic [Benincasa hispida]

2.6e-20

43.29

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Query:  DARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEY
        D+RV+ +     F   F   + +   + TG ++ +T  +   R LS    S    A +K    F+L              F ELFETLEKDLA AGRA+Y
Subjt:  DARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEY

Query:  NAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        NAP EQA FNFLARSLF  NP D+KL +DAPKLI KWVLF+L        P+   ELL    RL
Subjt:  NAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

TrEMBL top hits

e value

%identity

Alignment

A0A5A7VCB0 Allene oxide synthase 1

1.4e-19

42.68

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Query:  DARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEY
        D+RV+ +     F   F   + +   + TG ++ +T  +   R LS    S    A +K    F+L              F ELFETLEKDLA AGRAEY
Subjt:  DARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEY

Query:  NAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        NA  EQA FNFLARSLF  +P D+KL +DAPKLI+KWVLF+L        P+   ELL    RL
Subjt:  NAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

A0A6J1GGV7 allene oxide synthase 1, chloroplastic-like

1.6e-20

42.77

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        S D RV+ +     F   F   + +   + TG ++  T  +   R LS    S  S   +K    F+L             +F ELF+TLEKDLA  GRA
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        +YN P EQA FNFL RSL+ VNPA+TKL QDAPKLI KWVLF+L        P+   ELL H  RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

A0A6J1HCI8 allene oxide synthase 1, chloroplastic-like

7.4e-21

42.77

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        + D+RV+ +     F   F   + +   + TG ++  T  +   R LS    S      +K    F+L              F ELF+TLEKDLA  GRA
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        EYNAP EQA FNFLARSLF  NP DTKL +DAPKLI+KWVLF+L    R   P+   ELL    RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

A0A6J1K6Z3 allene oxide synthase 1, chloroplastic-like

9.6e-21

42.17

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        + D+RV+ +     F   F   + +   + TG ++  T  +   R LS    S      +K    F+L              F ELF+TLEKDLA  GRA
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        EYNAP EQA FNFLARSLF  NP DTKL +DAPKLI+KW+LF+L    R   P+   ELL    RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

U5YTX9 Allene oxide synthase

3.7e-20

42.68

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Query:  DARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEY
        D+RV+ +     F   F   + +   + TG ++ +T  +   R LS    S    A +K    F+L              F ELFETLEKDLA +GRAEY
Subjt:  DARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEY

Query:  NAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        NAP EQA FNFLARSLF  +P D+KL +DAPKLI+KWVLF+L        P+   ELL    RL
Subjt:  NAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

SwissProt top hits

e value

%identity

Alignment

K4BV52 Allene oxide synthase 1, chloroplastic

2.1e-17

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Query:  GKSFGML----RYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIK---FY-----RNFIL-----LFLELFETLEKDLATAGRAEYNAPDEQATF
        GKSF +L    + +   + TG  +  T+ +   R LS    S  + A +K   FY     RN ++      + ELFETLE +L+T G+A  NA ++QA F
Subjt:  GKSFGML----RYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIK---FY-----RNFIL-----LFLELFETLEKDLATAGRAEYNAPDEQATF

Query:  NFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        NFLARSL+ +NP DT+L  D PKLI KWVLF+L        P+   +L+ H  RL
Subjt:  NFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

P48417 Allene oxide synthase, chloroplastic

1.2e-15

35.8

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Query:  RVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAG--------IKFYRNFIL-----LFLELFETLEKDLATAGRAEYNA
        RV+ +  +  F   F M + +   + TG ++  T  +   R LS    S  +           IK  R++++      F +L E +E DLAT G+A +N 
Subjt:  RVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAG--------IKFYRNFIL-----LFLELFETLEKDLATAGRAEYNA

Query:  PDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        P EQA FNFL+R+ F V P DT L +DAP LISKWVLF L+       P+   E   H  RL
Subjt:  PDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

Q40778 Allene oxide synthase

3.5e-12

33.13

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Query:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA
        S + +V+ +  +  F   F + + +   + TG ++  T  +   R LS    S    A +K    F+L              + ELFE LE +LA  G+A
Subjt:  SRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRA

Query:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
         +N   EQA F FL R+ F+ NP +TKL   AP LIS WVLF L+       P   +E L H  RL
Subjt:  EYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

Q96242 Allene oxide synthase, chloroplastic

2.5e-13

36.43

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Query:  YQNSPVATGIHLILTNRSPIT---RSLSNRSSSSSSTAGIKFYRNFILLFLELFETLEKDLATAGRAEYNAPDEQATFNFLARSLFDVNPADTKLAQDAP
        Y  S   TG + IL+   P       L N       ++  + +  F   + ELF++LEK+L+  G+A++    +   FNFLAR+ +  NPADTKL  DAP
Subjt:  YQNSPVATGIHLILTNRSPIT---RSLSNRSSSSSSTAGIKFYRNFILLFLELFETLEKDLATAGRAEYNAPDEQATFNFLARSLFDVNPADTKLAQDAP

Query:  KLISKWVLFRLSLFSRTRPPQARRELLPH
         LI+KWVLF L        P+   E L H
Subjt:  KLISKWVLFRLSLFSRTRPPQARRELLPH

Q9LLB0 Allene oxide synthase 2, chloroplastic

1.4e-13

34.84

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Query:  GKSFGML----RYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEYNAPDEQATF
        GKSF +L    + +   + TG  +  T  +   R LS    S  +   +K    F+L              + ELFETL+K++   G   +N+  +QA F
Subjt:  GKSFGML----RYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFIL-------------LFLELFETLEKDLATAGRAEYNAPDEQATF

Query:  NFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL
        NFLARSLF VNP +TKL  D P LI KW+L +L        P+   ++L H  RL
Subjt:  NFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRL

Arabidopsis top hits

e value

%identity

Alignment

AT5G42650.1 allene oxide synthase

1.7e-14

36.43

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Query:  YQNSPVATGIHLILTNRSPIT---RSLSNRSSSSSSTAGIKFYRNFILLFLELFETLEKDLATAGRAEYNAPDEQATFNFLARSLFDVNPADTKLAQDAP
        Y  S   TG + IL+   P       L N       ++  + +  F   + ELF++LEK+L+  G+A++    +   FNFLAR+ +  NPADTKL  DAP
Subjt:  YQNSPVATGIHLILTNRSPIT---RSLSNRSSSSSSTAGIKFYRNFILLFLELFETLEKDLATAGRAEYNAPDEQATFNFLARSLFDVNPADTKLAQDAP

Query:  KLISKWVLFRLSLFSRTRPPQARRELLPH
         LI+KWVLF L        P+   E L H
Subjt:  KLISKWVLFRLSLFSRTRPPQARRELLPH

Sequences

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CDS sequence

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ATGAATGACGAGATAGTGTGGAAATACTCCAATCGAGGTGTGTTCACAGTGAAAAGTGCTTATTGGCTTGGGCTTAAAGTCAAGTTTTCTCGTGATGCACGAGTTCTGAC
AGTGAGAAGGTCAGGAGTTTTTGGAAAAAGTTTTGGGATGCTAAGGTACCAAAACTCACCGGTGGCTACAGGGATTCATCTTATATTGACCAATCGGAGCCCGATCACGA
GAAGCTTAAGCAACCGATCTTCTTCCTCCTCAAGCACCGCCGGGATAAAATTCTACCGAAATTTCATTCTACTTTTTTTGGAGCTTTTCGAGACTCTGGAGAAGGATTTG
GCCACCGCTGGTAGGGCCGAGTACAATGCTCCCGATGAACAGGCGACGTTTAATTTCTTGGCTCGGTCTCTCTTCGATGTTAATCCGGCGGATACCAAATTGGCCCAAGA
CGCTCCCAAATTGATTTCGAAATGGGTTCTGTTCCGGCTTAGCCTGTTCTCTCGGACTCGACCTCCCCAAGCTCGTCGAGAGCTCCTCCCCCACGACGGCCGGCTCCGTG
GACGTTAA

mRNA sequence

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Protein sequence

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MNDEIVWKYSNRGVFTVKSAYWLGLKVKFSRDARVLTVRRSGVFGKSFGMLRYQNSPVATGIHLILTNRSPITRSLSNRSSSSSSTAGIKFYRNFILLFLELFETLEKDL
ATAGRAEYNAPDEQATFNFLARSLFDVNPADTKLAQDAPKLISKWVLFRLSLFSRTRPPQARRELLPHDGRLRGR

Tan0021037 (gene) of Snake gourd v1 genome